Product Info Summary
SKU: | A00677Y771 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-PLC gamma (Y771) PLCG1 Antibody
View all PLC-gamma 1 Antibodies
SKU/Catalog Number
A00677Y771
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-PLC gamma (Y771) PLCG1 Antibody catalog # A00677Y771. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PLC gamma (Y771) PLCG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00677Y771)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PLC gamma1 around the phosphorylation site of Y771.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00677Y771 is reactive to PLCG1 in Human, Monkey, Mouse, Rat
Applications
A00677Y771 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
148.532kDa
Background of PLC-gamma 1
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Denis Tvorogov, J. Cell Sci., Feb 2005; 118: 601 - 610.
Brenda J. Irvin, Mol. Cell. Biol., Dec 2000; 20: 9149 - 9161.
Benoit Poulin, J. Biol. Chem., Feb 2000; 275: 6411.
Ichiro Nakamura, J. Cell Biol., Jan 2001; 152: 361.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of PLCG1 using Anti-Phospho-PLC gamma (Y771) PLCG1 Antibody (A00677Y771).
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°CClick image to see more details
Figure 3. Immunohistochemistry validation of PLCG1 using Anti-Phospho-PLC gamma (Y771) PLCG1 Antibody (A00677Y771).
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°CClick image to see more details
Figure 4. Immunohistochemistry validation of PLCG1 using Anti-Phospho-PLC gamma (Y771) PLCG1 Antibody (A00677Y771).
For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Phospho-PLC gamma (Y771) PLCG1 Antibody A00677Y771
Western Blot (WB) analysis of specific cells using Phospho-PLC gamma1 (Y771) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PLCG1 (Source: Uniprot.org, NCBI)
Gene Name
PLCG1
Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Weight
148.532kDa
Alternative Names
EC 3.1.4.11; gamma 1 (formerly subtype 148); monophosphatidylinositol phosphodiesterase; NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1; phospholipase C-148; Phospholipase C-gamma-1; Phospholipase C-II; PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; PLC148; PLC-148; PLCG1; PLCgamma 1; PLC-gamma 1; PLC-gamma-1; PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; PPLCA; triphosphoinosi PLCG1 NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1 phospholipase C gamma 1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1|1-phosphatidyl-D-myo-inositol-4,5-bisphosphate|PLC-148|PLC-gamma-1|inositoltrisphosphohydrolase|monophosphatidylinositol phosphodiesterase|phosphatidylinositol phospholipase C|phosphoinositidase C|phosphoinositide phospholipase C|phosphoinositide phospholipase C-gamma-1|phospholipase C, gamma 1 (formerly subtype 148)|phospholipase C-148|phospholipase C-II|triphosphoinositide phosphodiesterase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLCG1, check out the PLCG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLCG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PLC gamma (Y771) PLCG1 Antibody (A00677Y771)
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No publications found for A00677Y771
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